​CELL BIOLOGY OF INFLAMMATION

Activation of Rac1 and RhoA Preserve Corneal Endothelial Barrier Function. Ortega M.C., Santander-García D., Marcos-Ramiro B., Barroso S., Cox S., Jiménez-Alfaro I. and Millán J.

Invest Ophthalmol Vis Sci. 2016 Nov 1;57(14):6210-6222. doi: 10.1167/iovs.16-20031.

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A human cellular system for analyzing signaling during corneal endothelial barrier dysfunction. Santander-García D., Ortega M.C.,  Benito-Martínez S., Barroso S., Jiménez-Alfaro I. and Millán J.

Experimental Eye Research. 2016, Sept 30. http://dx.doi.org/10.1016/j.exer.2016.09.010

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García-Weber D. and Millán J. Parallels between single cell migration and barrier formation: the case of RhoB and Rac1 trafficking.

Small GTPases. 2016. Sep 6.  DOI: 10.1080/21541248.2016.1231655.

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Bernabé-Rubio M, Andrés G, Casares-Arias J, Fernández-Barrera J, Rangel L, Reglero-Real N, Gershlick DC, Fernández JJ, Millán J, Correas I, Miguez DG, Alonso MA. Novel role for the midbody in primary ciliogenesis by polarized epithelial cells.

Journal of Cell Biology. 2016 Aug 1;214(3):259-73. doi: 10.1083/jcb.201601020.

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Marcos-Ramiro B, García-Weber D, Barroso S, Feito J, Ortega MC, Cernuda-Morollón E, Reglero-Real N, Fernández-Martín L, Durán MC, Alonso MA, Correas I, Cox S, Ridley AJ, Millán J. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border.

Journal of Cell Biology. 2016 May 2. pii: jcb.201504038.

 

Reglero-Real N, García-Weber D, Millán J. Cellular Barriers after Extravasation: Leukocyte Interactions with Polarized Epithelia in the Inflamed Tissue.

Mediators of Inflammation. 2016:7650260. doi: 10.1155/2016/7650260. Epub 2016 Jan 28. Review.

 

Rodríguez-Fraticelli AE, Bagwell J, Bosch-Fortea M, Boncompain G, Reglero-Real N, García-León MJ, Andrés G, Toribio ML, Alonso MA, Millán J, Perez F, Bagnat M, Martín-Belmonte F. Developmental regulation of apical endocytosis controls epithelial patterning in vertebrate tubular organs.

Nature Cell Biology. 2015 Mar;17(3):241-50. doi: 10.1038/ncb3106.

 

Reglero-Real R, Álvarez-Varela A, Cernuda-Morollón E, Feito J, Marcos-Ramiro B,Fernández-Martín L, Gómez-Lechón MJ, Muntané J, Sandoval P, Majano PL, Correas I, Alonso MA and Millán J.

Apicobasal polarity controls lymphocyte adhesion to hepatic epithelial cells.

Cell Reports; 2014. Sep 25;8(6):1879-93. DOI: http://dx.doi.org/10.1016/j.celrep.2014.08.007.

 

Marcos-Ramiro B, García-Weber D, Millán J.

TNF-induced endothelial barrier disruption: beyond actin and Rho.

Thrombosis and Haemostasis. 2014, http://dx.doi.org/10.1160/TH14A04A0299.

Marcos-Ramiro B, Oliva-Nacarino, P., Serrano-Pertierra, E., Blanco-Gelaz, M.A., Weksler,  B.B., Romero I.A., Couraud, P.O., Tuñon, A., Lopez-Larrea, C., Millán, J (C. Author)., and Cernuda, E (C. Author).

Microparticles in multiple sclerosis and clinically isolated syndrome: effect on endothelial barrier function.

BMC Neuroscience. 2014, doi:10.1186/1471-2202-15-110.

 

Aranda J.F., Reglero-Real N., Marcos-Ramiro B. Ruiz-Sáenz A., Fernández-Martín L., Bernabé-Rubio M., Kremer L., Ivetic A., Ridley A.J., Correas I., Alonso M.A. and Millán, J.

MYADM controls endothelial barrier function through ERM-dependent regulation of ICAM-1
expression.

Molecular Biology of the Cell. 2013, Feb;24(4):483-94.

 

Ruiz-Saenz A, van Haren J, Laura Sayas C, Rangel L, Demmers J, Millán J, Alonso MA, Galjart N, Correas I.

Protein 4.1R binds to CLASP2 and regulates dynamics, organization and attachment of microtubules to the cell cortex.

Journal of Cell Science. 2013 Oct 15;126(Pt 20):4589-601. doi: 10.1242/jcs.120840.

 

Fernández-Martín, L. Marcos-Ramiro, B., Bigarella, CL. Graupera,  M. Cain, RJ.  Reglero-Real, N., Jiménez A, Cernuda-Morollón, E., Correas, I. Cox, S., Ridley, AJ and Millán J.

Crosstalk between Reticular Adherens Junctions and PECAM-1 Regulates Endothelial Barrier Function.

Arteriosclerosis, Thrombosis and Vascular Biology. 2012. 32(8):e90-e102.          

 

Reglero-Real, N., Marcos-Ramiro, B. and Millán J. 

Endothelial membrane reorganization during leukocyte extravasation. 

Cell Molecular Life Sciences. 2012.   10.1007/s00018-012-0987-4. [Epub ahead of print]    

 
Ruiz-Sáenz, A. Kremer, L,Alonso, M.A. Millán, J and  Correas, I. 

Protein 4.1R regulates cell migration and IQGAP1 recruitment to the leading edge. 

Journal Cell Science; 2011. 124(Pt 15):2529-38.
 

Aranda JF, Reglero-Real N, Kremer L, Marcos-Ramiro B, Ruiz-Sáenz A, Calvo M, Enrich C, Correas I, Millán J (C. Author, equal contribution), Alonso MA (C. Author, equal contribution). 

MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration.

Molecular Biology of the Cell. 2011. 22(8):1252-62.

 

Cernuda-Morollón, E, Gharbi, S. and Millán, J.

Discriminating between the paracellular and transcellular routes of transmigration. “Studying T cell trafficking”.

Methods in Molecular Biology.2010; 616:69-82.

 

Cernuda-Morollón E, Millán J, Shipman M, Marelli-Berg FM, Ridley AJ.

Rac activation by the T-cell receptor inhibits T cell migration

PLoS One. 2010. 5(8):e12393.

 

Millán J (Corresponding author), Cain, R. Reglero-Real, N, Bigarella, C. Marcos-Ramiro, B, Fernández-Martín, L. Correas I. and Ridley A.J.

 Adherens junctions connect stress fibers between adjacent endothelial cells.

BMC Biology. 2010. 8:11.

 

Antón, O., Batista, A., Millán, J., Puertollano, R., Correas, I and Alonso M.A.

An Essential Role for the MAL Protein in Targeting Lck to the Plasma Membrane of Human T Lymphocytes.

Journal Experimental Medicine 2008. 205, 3201-13

 

Graupera, M., Guillermet-Guibert, J., Foukas, L.C., Salpekar, A., Pearce, W., Meek, S.,  Phng, L.P., Millán, J., Cutillas, P.R., Reynolds, A.R., Marinen, T. Smith, A.J.H., Ruhrberg, C., Gerhardt, H. and Vanhaesebroeck, B.

Isoform-selective role of the p110a PI 3-kinase in angiogenesis through regulation of endothelial cell migration.

Nature. 2008. 453, 662-666.

 

Millán J., Hewlett L., Glyn, M, Toomre, D., Clark, P. and Ridley A.J.

Lymphocyte transcellular migration occurs through recruitment of endothelial ICAM-1 to caveola and F-actin -rich domains.

Nature Cell Biology. 2006. 8, 113-123

                                                                                                                   

Millán J., Williams, L. and Ridley A.J.

An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration

Methods in Enzymology. 2006. 406, 643-55                                              

 

Millán, J and Ridley, A.J.

Rho GTPases and leucocyte-induced endothelial remodelling.

Biochemical Journal. 2005. 385:329-337    

 

Batista, A., Millán, J. (joint first author), Mittelbrunn, M, Sanchez-Madrid, F., Alonso M.A.:

Recruitment of Transferrin Receptor to Immunological Synapse in Response to TCR Engagement

Journal of Immunology. 2004. 172:6709-6714.                                         

 

Millán, J., De Marco MC, Qaidi M, Batista A, Martin-Belmonte F, Alonso MA.

Isolation of lipid raft-associated proteolipids.

Methods in Molecular Biology, 2003. 228:223-230.            

 

Millán, J., Montoya, M.C., Sancho, D., Sanchez-Madrid, F. and Alonso, M.A.

Lipid rafts mediate biosynthetic transport to the T lymphocyte uropod subdomain and are necessary for uropod integrity and function.

Blood, 2002. 99, 978-984.    

 

M. A. Alonso. M.A. and Millan J.

The role of lipid rafts in signalling and membrane trafficking in T lymphocytes.

Journal of Cell Science.  2001. 114, 3957-3965